符军
符军，教授，博士生导师，山东省泰山学者特聘专家。现任上海市生物医药技术研究院生物制造研究组特聘教授、山东大学微生物改造技术全国重点实验室教授。
研究方向
DNA同源重组机理研究，基因克隆技术开发以及位点特异性重组技术的开发。
基因转座技术在原核和真核细胞中的应用。
基因打靶技术和BAC转基因技术研究；转基因动物。
细菌次生代谢产物基因簇的克隆，修饰和异源表达；药物开发。
病毒基因组DNA文库构建和重组疫苗开发。
教育经历
2004/03－2012/04 德累斯顿工业大学，生物技术博士
1998/10－2001/07 德累斯顿工业大学，林学硕士
1987/09－1991/07 西北农林科技大学，林学学士
工作经历
2026/03－至今 上海市生物医药技术研究院，生物制造研究组，特聘教授
2025/01－至今 山东大学，微生物改造技术全国重点实验室（研究院），教授
2018/01－2024/12 山东大学，微生物技术国家重点实验室（研究院），教授
2014/04－2017/12 山东大学，生命科学学院，教授
2004－2014 德累斯顿工业大学，生物技术中心，研究员
2002－2004 基因桥公司（Gene Bridges GmbH），科学家
1991－1998 西北农林科技大学，讲师/助理研究员
荣誉获奖
2022年 山东省泰山学者特聘专家（团队专项负责人，再次获评）
2021年 山东省科学技术进步奖二等奖（证书编号：JB2021-2-33-R09，第9完成人）
2019年 高等学校科学研究优秀成果奖（科学技术）自然科学奖二等奖——“Red/ET基因组编辑与微生物资源发掘”（证书编号：1308-105，第4完成人）
2018年 山东省泰山学者特聘专家
主持的科研项目
1. 应用自身噬菌体重组蛋白建立枯草芽孢杆菌DNA同源重组系统（国家自然科学基金面上项目, 31570094，2016.1－2019.12，62万元；第一主持人）
2. 磁小体基因簇的克隆及应用于肿瘤靶向治疗（国家国际科技合作专项, 2015DFE32850，2015.4－2018.3，157 万元；第一主持人）
3. 杀线虫根际靶向生防工程菌的人工构建 （山东省自然科学基金重大基础研究项目，ZR2018ZC2261，2018.6－2020.12，120万元；第一主持人）
4. 放线菌底盘适配性机理与产物高产机制研究 – 课题三：高效基因编辑与异源表达技术开发 （国家重点研发计划2018YFA0900400，2019.7-2024.6，533万元；课题负责人）
5. 非天然噬菌体的设计合成 – 课题二：基因编码非天然氨基酸扩展噬菌体蛋白功能 （国家重点研发计划2019YFA0904000，2020.1-2024.12，90万元；课题骨干）
6. 高效基因编辑与异源表达技术开发(补助) （山东省科技厅/2018YFA0900403 (补助)，57万）
7. 高效基因编辑与异源表达技术开发(奖励）（山东省科技厅/2018YFA0900403 (奖励)，8.12万）
8. 山东泰和水处理科技股份有限公司横向课题“辅酶Q10高产菌株的遗传改造及发酵工艺优化”（2021年6月10日-2023年12月31日，75万元；第一主持人）
9. 异种器官移植人源化猪模型的构建以及人源化肾脏器官再生技术的探索 （山东省自然科学基金重大基础研究项目，ZR2021ZD39，2022.1－2024.12，200万元；第一主持人）
10. 齐鲁动物保健品有限公司横向课题“鸡新城疫病毒的定点改造”（2022年4月15日-2023年03月21日，20万元；第一主持人）
11. 基因工程生防抗体研发技术体系研究（军委后勤保障部卫生局，应用基础研究，项目编号：145AHQ080013000X-3；2022年6月 – 2025年12月，100万元；协作）
12. 西藏自治区科技计划项目，西藏传统发酵食品有害微生物控制，项目编号：XZ202601ZY0071，2026.01月至2028.12，126万元，项目负责人：符军。Science and Technology Projects of Xizang Autonomous Region, China” (XZ202601ZY0071)。
发表论文（82篇论文影响因子超过650，被引用超过6200次，h-index=38）
13. Yuxin Cao#, Linfeng Wang#, Cailing Ren#, Chenxi Li, Yang Liu, Tong Zhang, Mengjiao Luo, Pan Zhao, Ruijuan Li*, Jun Fu*, Jia Yin*. Oral supplementation of Pediococcus pentosaceus ameliorates hyperuricemia and alcohol-induced injury. Applied and Environmental Microbiology (Appl Environ Microbiol). 2026, 92(4):e0024626. Accepted on Feb 16, 2026. Published online: Feb 16, 2026. DOI: 10.1128/aem.00246-26.(通讯作者)
14. Runyu Yang, Yitong Shi, Jinyu Bian, Mengxiao Sun, Junchi Ma, Cailing Ren, Xueyan Feng, Yun Wang, Yuanhui Yang, Jianqiang Zhou*, Jun Fu*, and Ruijuan Li*. (2025) Genetically engineered Pseudomonas aeruginosa with lipase regulation for production of rhamnolipids from waste frying oil. Frontiers in Microbiology (Front Microbiol). 2025, 16:1691217. Accepted on Oct 14, 2025. Published online: Nov 20, 2025. (通讯作者)
15. Shiqing Gao#, Xiuling Wang#, Runyu Yang, Zhilong Bian, Daojing Yong, Shouying Xu, Liujie Huo, Qiang Tu, Youming Zhang, Jun Fu*, and Ruijuan Li*.(2025) Efficient genome engineering in Agrobacterium tumefaciens C58 using recombineering assisted by CRISPR/Cas9. Journal of Biotechnology (Short communication). 2025, 406: 99-104. Accepted on July 03, 2025. Published online: July 04, 2025. DOI: 10.1016/j.jbiotec.2025.07.005. (通讯作者)
16. Xiuling Wang#, Shiqing Gao#, Jun Fu*, Ruijuan Li*(2025). Heterologous expression of the nitrogen-fixing gene cluster from Paenibacillus polymyxa in Bacillus subtilis. Microorganisms (Brief report). 2025, 13(6), 1320. Accepted on June 5, 2025. Published online: June 6, 2025. DOI: 10.3390/microorganisms13061320. (通讯作者)
17. Xiuling Wang, Ping Lin, Qiyao Shen, Xueyan Feng, Shouying Xu, Qijun Zhang, Yang Liu, Cailing Ren, Daojing Yong, Qiong Duan, Liujie Huo, Youming Zhang, Gang Li*, Jun Fu*, Ruijuan Li* (2025). A highly efficient heterologous expression platform to facilitate the production of microbial natural products in Streptomyces. Microbial Cell Factories (Microb Cell Fact). 2025, 24(1):105. Accepted on Apr 16, 2025. Published online: May 14, 202. DOI: 10.1186/s12934-025-02722-z. (通讯作者) (第一标注项目：2018YFA0900400 放线菌底盘)
18. Xiaoling Yan, Xiaoli Zhang, Ling Zhou, Xiaoya Zhao, Qianniu Li, Tian Lan*, Jun Fu*, Jingyun Ma* (2025). Effects of SADS-CoV accessory proteins NS3a, NS7a, and NS7b on viral pathogenicity: A multi-omics investigation. iMetaOmics e70015. Published online: April 06, 2025. doi.org/10.1002/imo2.70015 (通讯作者)
19. Xiuling Wang, Zhuo Zhang, Jun Fu*, Ruijuan Li* (2025). Genomics-driven discovery of plantariitin A, a novel lipopeptide in Burkholderia plantarii DSM9509. Molecules (Molecules) (Communication). 2025, 30(4): 868. Accepted on Feb 10, 2025. Published online: Feb 14, 2025. DOI:10.3390/molecules30040868. (通讯作者)
20. Wentao Zheng#, Yandong Xia#, Xue Wang, Shiqing Gao, Diao Zhou, Vinothkannan Ravichandran, Chanjuan Jiang, Qiang Tu, Yulong Yin, Youming Zhang, Jun Fu*, Ruijuan Li*, Jia Yin* (2023). Precise genome engineering in Pseudomonas using phage-encoded homologous recombination and Cascade-Cas3 system. Nature Protocols (Nat Protoc). 2023, 18(9): 2642-2670. Accepted on May 11, 2023. Published online: Aug 25, 2023. DOI: 10.1038/s41596-023-00856-1. (通讯作者) (第一标注项目：2019YFA0904000 人工噬菌体)
21. Qingshu Liu#, Ruijuan Li#,*, Hongbo Shi, Runyu Yang, Qiyao Shen, Qingwen Cui, Xiuling Wang, Aiying Li, Youming Zhang*, Jun Fu* (2023). A recombineering system in Bacillus subtilis based on the native phage recombinase pair YqaJ/YqaK. Engineering Microbiology. 2023, 3(3): 100099. Accepted on Jun 09, 2023. Published online: July 19, 2023. Available online: June 21, 2023. DOI: 10.1016/j.engmic.2023.100099. (通讯作者) (第一标注项目：2019YFA0904000 人工噬菌体)
22. Ruijuan Li, Aiying Li, Youming Zhang, Jun Fu*(2023). The emerging role of recombineering in microbiology (Review). Engineering Microbiology. 2023, 3(3): 100097. Accepted on May 10, 2023. Published online: June17, 2023. DOI: 10.1016/j.engmic.2023.100097. (通讯作者) (第一标注项目：2019YFA0904000 人工噬菌体)
23. Kai Gong, Maoqin Wang, Qiong Duan, Gang Li, Daojing Yong, Cailing Ren, Yue Li, Qijun Zhang, Tao Sun, Huanyun Zhang, Qiang Tu, Changsheng Wu, Jun Fu, Aiying Li, Chaoyi Song*, Youming Zhang*, Ruijuan Li*(2022). High-yield production of FK228 and new derivatives in a Burkholderia chassis. Metabolic Engineering (Metab Eng). 2023, 75(2023): 131-142. Accepted on Dec 12, 2022. Available online Dec 15, 2022. Published online: Dec 22, 2022. DOI: 10.1016/j.ymben.2022.12.002. (第一标注项目：2019YFA0904000 人工噬菌体)
24. Daojing Yong , Yue Li, Kai Gong, Yingying Yu, Shuai Zhao, Qiong Duan, Cailing Ren, Aiying Li, Jun Fu, Jinfeng Ni*, Youming Zhang*, Ruijuan Li * (2022) “Biocontrol of strawberry gray mold caused by Botrytis cinerea with the termite associated Streptomyces sp. sdu1201 and actinomycin D” Front Microbiol . 2022 Nov 4;13:1051730. doi: 10.3389/fmicb.2022.1051730. eCollection 2022. (第一标注项目：2019YFA0904000 人工噬菌体)
25. Wentao Zheng, Yandong Xia, Xue Wang, Shiqing Gao, Diao Zhou, JunFu, Ruijuan Li, Jia Yin (2022) “Cascade-Cas3 facilitates high-accuracy genome engineering in Pseudomonas using phage-encoded homologous recombination” Engineering Microbiology, 2022 Dec.; 2 (4): 100046; (通讯作者) (第一标注项目：2019YFA0904000 人工噬菌体)
26. Guanming Shao, Zi Xie, Ming Liang, Yaxin Liu, Chaoyi Song, Keyu Feng, Xinheng Zhang, Wencheng Lin, Jun Fu, Qingmei Xie (2022) “Efficacy of recombinant Newcastle disease virus expressing HA protein of H9N2 Avian influenza virus in respiratory and intestinal tract” Poult Sci. 2022 Jul 26;101(12):102078.doi: 10.1016/j.psj.2022.102078. Online ahead of print.
27. Gong K, Yong D, Fu J, Li A, Zhang Y, Li R. (2022) “Diterpenoids from Streptomyces: Structures, Biosyntheses and Bioactivities.” Chembiochem. 2022 Sep 5;23(17):e202200231. doi: 10.1002/cbic.202200231. Epub 2022 Jun 20. (Review.)
28. Liyi Chen, Xinheng Zhang, Guanming Shao, Yangyang Shao, Zezhong Hu, Keyu Feng, Zi Xie, Hongxin Li, Weiguo Chen, Wencheng Lin, Hengxing Yuan, Hailong Wang, Jun Fu, and Qingmei Xie (2022) “Construction and Evaluation of Recombinant Pseudorabies Virus Expressing African Swine Fever Virus Antigen Genes” Frontiers in Veterinary Science 2022; 9: 832255.
29. Hengxing Yuan, Yaoyao Zheng, XiaolingYan, Hailong Wang,Youming Zhang, Jingyun Ma, Jun Fu (2022) “Direct cloning of a herpesvirus genome for rapid generation of infectious BAC clones” Journal of Advanced Research. 2023, 43:97-107. (通讯作者，12.822) (第一标注项目：2019YFA0904000 人工噬菌体)
30. Anne Lanois-Nouri, Lucile Pantel, Jun Fu, Jessica Houard, Jean-Claude Ogier, Yury S Polikanov, Emilie Racine, Hailong Wang, Sophie Gaudriault, Alain Givaudan, Maxime Gualtieri. （2022） “The Odilorhabdin Antibiotic Biosynthetic Cluster and Acetyltransferase Self-Resistance Locus Are Niche and Species Specific” mBio. 2022 Jan 11;13(1):e0282621.
31. Zhilong Bian, Shanshan Li, Runyu Yang, Jia Yin, Youming Zhang, Qiang Tu , Jun Fu , Ruijuan Li (2022) “Development of a New Recombineering System for Agrobacterium Species” Applied and Environmental Microbiology. 2022 Mar 8;88(5):e0249921. doi: 10.1128/aem.02499-21. (通讯作者，5.005) (第一标注项目：2019YFA0904000 人工噬菌体)
32. Qiyao Shen, Guangzhi Dai, Aiying Li, Yang Liu, Guannan Zhong, Xiaoju Li, Xiangmei Ren, Haiyan Sui, Jun Fu, Nianzhi Jiao, Youming Zhang, Xiaoying Bian, Haibo Zhou. (2021) “Genome-Guided Discovery of Highly Oxygenated Aromatic Polyketides, Saccharothrixins D-M, from the Rare Marine Actinomycete Saccharothrix sp. D09” J Nat Prod . 2021 Nov 26;84(11):2875-2884. (第二标注项目：2018YFA0900400 放线菌底盘)
33. Chanjuan Jiang, Haibo Zhou, Hongluan Sun, Ruoting He, Chaoyi Song, Tianqi Cui, Ji Luan, Jun Fu, Youming Zhang, Nianzhi Jiao, Hailong Wang (2021) “Establishing an efficient salinomycin biosynthetic pathway in three heterologous Streptomyces hosts by constructing a 106-kb multioperon artificial gene cluster” Biotechnol Bioeng . 2021 Dec;118(12):4668-4677 (第一标注项目：2018YFA0900400 放线菌底盘)
34. 李瑞娟 赵晓雨 杨润雨 刘洋 颜富 王海龙 张友明 符军 (2021) “噬菌体重组酶介导的DNA同源重组工程” 微生物学通报. Sep. 20, 2021, 48(9): 3230−3248. DOI: 10.13344/j.microbiol.china.210402. (通讯作者) (第一标注项目：2019YFA0904000 人工噬菌体；第二标注项目：2018YFA0900400 放线菌底盘)
35. Ruijuan Li, Hongbo Shi, Xiaoyu Zhao, Xianqi Liu , Qiong Duan, Chaoyi Song, Hanna Chen, Wentao Zheng, Qiyao Shen, Maoqin Wang, Xue Wang, Kai Gong, Jia Yin, Youming Zhang, Aiying Li, Jun Fu. (2021) “Development and application of an efficient recombineering system for Burkholderia glumae and Burkholderia plantarii” Microbial Biotechnology. 2021 Jul;14(4):1809-1826. doi: 10.1111/1751-7915.13840. Epub 2021 Jun 30. (通讯作者) (第一标注项目：2018YFA0900400 放线菌底盘；第二标注项目：2019YFA0904000 人工噬菌体)
36. Wentao Zheng, Xue Wang, Yuwei Chen, Yachao Dong, Diao Zhou, Ruxin Liu, Haibo Zhou, Xiaoying Bian, Hailong Wang, Qiang Tu, Vinothkannan Ravichandran, Youming Zhang, Aiying Li, Jun Fu, Jia Yin (2021) “Recombineering facilitates the discovery of natural product biosynthetic pathways in Pseudomonas parafulva” Biotechnology Journal. 2021, doi: 10.1002/biot.202000575. (通讯作者) (第一标注项目：2018YFA0900400 放线菌底盘；第二标注项目：2019YFA0904000 人工噬菌体)
37. Chaoyi Song, Ji Luan, Ruijuan Li, Chanjuan Jiang, Yu Hou, Qingwen Cui,Tianqi Cui, Long Tan, Zaichao Ma, Ya-Jie Tan, A. Francis Stewart, Jun Fu,Youming Zhang and Hailong Wang (2020) “RedEx: a method for seamless DNA insertion and deletion in large multimodular polyketide synthase gene clusters” Nucleic Acids Research, 2020, doi: 10.1093/nar/gkaa956 (第一标注项目：2018YFA0900400 放线菌底盘)
38. Ji Luan, Zhen Li, Hailong Wang, Jun Fu, Youming Zhang (2021) “In vitro Assay Revealed Mismatches between Guide RNA and Target DNA can Enhance Cas9 Nuclease Activity” Current Chinese Science, Volume 1 , Issue 1 , 2021
39. Deepthi Ashokkumar, Qinyu Zhang, Christian Much, Anita S Bledau, Ronald Naumann, Dimitra Alexopoulou, Andreas Dahl, Neha Goveas, Jun Fu, Konstantinos Anastassiadis, A Francis Stewart, Andrea Kranz (2020) “MLL4 is required after implantation, whereas MLL3 becomes essential during late gestation” Development. 2020 Jun 17;147(12):dev186999.doi: 10.1242/dev.186999.
40. Ruxin Liu, Yuhan Zhang, Huifang Guo, Ning Li, Baiyu Wang, Kaiyue Tian, Zeng Wang, Xia Yang, Yongtao Li, Hailong Wang, Youming Zhang, Jun Fu, Jun Zhao (2020) “The increased virulence of hypervirulent fowl adenovirus 4 is independent of fiber-1 and penton” Research In Veterinary Science. 2020 Aug;131:31-37. doi: 10.1016/j.rvsc.2020.04.005. Epub 2020 Apr 6. (通讯作者)
41. Muhammad Nazeer Abbasi, Jun Fu, Xiaoying Bian, Hailong Wang, Youming Zhang, Aiying Li (2020) “Recombineering for Genetic Engineering of Natural Product Biosynthetic Pathways” Trends Biotechnol. 2020 Jan 20. pii: S0167-7799(19)30313-0. doi: 10.1016/j.tibtech.2019.12.018. (Review) (第一标注项目：2018YFA0900400 放线菌底盘)
42. Chen H, Bian Z, Ravichandran V, Li R, Sun Y, Huo L, Fu J, Bian X, Xia L, Tu Q, Zhang Y. (2019) “Biosynthesis of polyketides by trans-AT polyketide synthases in Burkholderiales” Crit Rev Microbiol. 2019 Mar;45(2):162-181. doi: 10.1080/1040841X.2018.1514365. (Review)
43. Jia Yin, Wentao Zheng, Yunsheng Gao, Chanjuan Jiang, Hongbo Shi, Xiaotong Diao, Shanshan Li, Hanna Chen, Hailong Wang, Ruijuan Li, Aiying Li, Liqiu Xia, Yulong Yin, A. Francis Stewart*, Youming Zhang* and Jun Fu*.（2019）“Single-Stranded DNA-Binding Protein and Exogenous RecBCD Inhibitors Enhance Phage-Derived Homologous Recombination in Pseudomonas” iScience. 2019 Apr 26;14:1-14. doi: 10.1016/j.isci.2019.03.007. (通讯作者)
44. Chaoyi Song, Ji Luan, Qingwen Cui, Qiuyue Duan, Zhen Li, Yunsheng Gao, Ruijuan Li, Aiying Li, Yue-Mao Shen, Yue-Zhong Li, Adrian Francis Stewart, Youming Zhang*, Jun Fu*, and Hailong Wang* （2019）“Enhanced heterologous spinosad production from a 79-kb synthetic multioperon assembly” ACS Synthetic Biology, 2019, 8, 1, 137-147 (通讯作者)
45. Fangnan Yu, Xiaoshu Jing, Xiaochen Li, Hailong Wang, Hanna Chen, Lin Zhong, Jia Yin, Deng Pan, Yulong Yin, Jun Fu, Liqiu Xia, Xiaoying Bian, QiangTu*, Youming Zhang*. (2019) “Recombineering Pseudomonas protegens CHA0: An innovative approach that improves nitrogen fixation with impressive bactericidal potency.” Microbiol Res. 2019 Jan;218:58-65.
46. Ruijuan Li, Linda Helbig, Jun Fu, Xiaoying Bian, Jennifer Herrmann, Michael Baumann, A. Francis Stewart, Rolf Müller, Aiying Lia, Daniel Zips, and Youming Zhang (2019) “Expressing Cytotoxic Compounds in Escherichia coli Nissle 1917 for Tumor-targeting Therapy.” Res Microbiol. Volume 170, Issue 2, March 2019, Pages 74-79.
47. Gulce Itir Percin, Jiri Eitler, Andrea Kranz, Jun Fu, Jeffrey W. Pollard, Ronald Naumann & Claudia Waskow (2018) “CSF1R regulates the dendritic cell pool size in adult mice via embryo-derived tissue-resident macrophages” Nature Communications. 9(1):5279.
48. Yuhan Zhang, Ruxin Liu, Kaiyue Tian, Zeng Wang, XiaYang, Dongsheng Gao, Youming Zhang, Jun Fu*, Hailong Wang*, Jun Zhao* （2018）“Fiber2 and hexon genes are closely associated with the virulence of the emerging and highly pathogenic fowl adenovirus 4” Emerging Microbes & Infections. 7(1):199. doi: 10.1038/s41426-018-0203-1. (通讯作者)
49. Xiaoshu Jing, Qingwen Cui, Xiaochen Li, Jia Yin, Vinothkannan Ravichandran, Deng Pan, Jun Fu, Qiang Tu, Hailong Wang*, Xiaoying Bian*, Youming Zhang*. (2018) “Engineering Pseudomonas protegens Pf-5 to improve its antifungal activity and nitrogen fixation.” Microbial Biotechnology. doi: 10.1111/1751-7915.13335.
50. Jing Liu, Philip Boehme, Wenli Zhang, Jun Fu, Roma Yumul, Kemal Mese, Raphael Tsoukas, Manish Solanki, Michael Kaufmann, Ruirui Lu, Achim Schmidtko, A. Francis Stewart, André Lieber & Anja Ehrhardt*. (2018) “Human adenovirus type 17 from species D transduces endothelial cells and human CD46 is involved in cell entry.” Scientific Reports. 8(1):13442. doi: 10.1038/s41598-018-31713-x.
51. Wenli Zhang，Jun Fu and Anja Ehrhardt*. (2018) “Novel vector construction based on alternative adenovirus types via homologous recombination.” Human Gene Therapy Methods. 29(3):124-134
52. Xue Wang, Haibo Zhou1, Hanna Chen, Xiaoshu Jing, Wentao Zheng, Ruijuan Li, Tao Sun, Jiaqi Liu, Jun Fu, Liujie Huo, Yue-zhong Li, Yuemao Shen, Xiaoming Ding, Rolf Müller, Xiaoying Bian*, and Youming Zhang*.(2018) “Discovery of recombinases enables genome mining of cryptic biosynthetic gene clusters in Burkholderiales species”. Proceedings of the National Academy of Sciences of the United States of America. 15(18):E4255-E4263.
53. Xiaokun Xu, Haibo Zhou, Yang Liu, Xiaotong Liu, Jun Fu, Aiying Li, Yue-zhong Li, Yuemao Shen , Xiaoying Bian* and Youming Zhang*. (2018) Heterologous Expression Guides Identification of the Biosynthetic Gene Cluster of Chuangxinmycin, an Indole Alkaloid Antibiotic. Journal of Natural Products. 81(4):1060-1064.
54. Hailong Wang, Zhen Li，Ruonan Jia，Jia Yin, Aiying Li，Liqiu Xia，Yulong Yin, Rolf Müller, Jun Fu*, A. Francis Stewart* and Youming Zhang* (2018) “ExoCET: Exonuclease in vitro assembly combined with RecET recombination for highly efficient direct DNA cloning from complex genomes” Nucleic Acids Research. 2018 Mar 16;46(5):e28. doi: 10.1093/nar/gkx1249. (通讯作者)
55. Debojyoti Chakraborty, Maciej Paszkowski-Rogacz, Nicolas Berger, Li Ding, Jovan Mircetic, Jun Fu, Vytautas Iesmantavicius, Chunaram Choudhary, Konstantinos Anastassiadis, A. Francis Stewart, Frank Buchholz （2017） “IncRNA Panct1 Maintains Mouse Embryonic Stem Cell Identity by Regulating TOBF1 Recruitment to Oct-Sox Sequences in Early G1” Cell Reports. 21, 3012–3021
56. Oliver Baker, Sarah Tsurkan, Jun Fu, Barbara Klink, Andreas Rump, Mandy Obst, Andrea Kranz, Evelin Schröck, Konstantinos Anastassiadis and A. Francis Stewart (2017) “The contribution of homology arms to nuclease-assisted genome engineering.” Nucleic Acids Research 2017 Jun 5. doi: 10.1093/nar/gkx497.
57. Wenli Zhang, Jun Fu, Jing Liu, Hailong Wang, Maren Schiwon, Sebastian Janz, Lukas Schaffarczyk, Lukas von der Goltz, Eric Ehrke-Schulz, Johannes Dörner, Manish Solanki, Philip Boehme, Thorsten Bergmann, Andre Lieber, Chris Lauber, Andreas Dahl, Andreas Petzold, Youming Zhang, A. Francis Stewart and Anja Ehrhardt (2017) “An Engineered Virus Library as a Resource for the Spectrum-wide Exploration of Virus and Vector Diversity” Cell Reports. 2017 May 23;19(8):1698-1709. (并列第一作者)
58. Xiaoying Bian, Biao Tang, Yucong Yu, Qiang Tu, Frank Gross, Hailong Wang, Aiying Li, Jun Fu, Yuemao Shen , Yue-zhong Li, A. Francis Stewart, Guoping Zhao, Xiaoming Ding, Rolf Müller, and Youming Zhang (2017) “Heterologous Production and Yield Improvement of Epothilones in Burkholderiales Strain DSM 7029” ACS Chemical Biology. 2017 May 18. doi: 10.1021/acschembio.7b00097.
59. Müge Akpınar, Mathias Lesche, Grigorios Fanourgakis, Jun Fu, Konstantinos Anasstasiadis, Andreas Dahl and Rolf Jessberger (2017) “TDRD6 mediates early steps of spliceosome maturation in primary spermatocytes” PLOS Genetics 2017 Mar 6;13(3):e1006660. doi: 10.1371/journal.pgen.1006660
60. Yin J, Wang H, Li R, Ravichandran V, Bian X, Li A, Tu Q, Francis Stewart A, Fu J, Zhang Y. (2017) “A Practical Guide to Recombineering in Photorhabdus and Xenorhabdus” Current Topics in Microbiology and Immunology. 2017 Feb 3. doi: 10.1007/82_2016_57. (通讯作者)
61. Sivaraman Subramaniam, Axel Erler, Jun Fu, Andrea Kranz, Jing Tang, Mohanraj Gopalswamy, Saminathan Ramakrishnan, Adrian Keller, Guido Grundmeier, Daniel Müller, Michael Sattler & A. Francis Stewart (2016) "DNA annealing by Redβ is insufficient for homologous recombination and the additional requirements involve intra- and inter-molecular interactions" Scientific Reports. 2016 Oct 6;6:34525. doi: 10.1038/srep34525.
62. Qingshu Liu, Qiyao Shen, Xiaoying Bian, Hanna Chen, Jun Fu, Hailong Wang, Ping Lei, Zhaohui Guo, Wu Chen, Dingjun Li & Youming Zhang. (2016) "Simple and rapid direct cloning and heterologous expression of natural product biosynthetic gene cluster in Bacillus subtilis via Red/ET recombineering" Scientific Reports. 2016 Sep 30;6:34623. doi: 10.1038/srep34623.
63. Hu, S., Liu, Z., Zhang, X., Zhang, G., Xie, Y., Ding, X., Mo, X., Stewart, A.F., Fu, J., Zhang, Y. and Xia, L. (2016) "Cre/loxP plus BAC: a strategy for direct cloning of large DNA fragment and its applications in Photorhabdus luminescens and Agrobacterium tumefaciens." Scientific Reports. 2016 Jul 1;6:29087. doi: 10.1038/srep29087. (通讯作者)
64. Hailong Wang, Zhen Li, Ruonan Jia, Yu Hou, Jia Yin, Xiaoying Bian, Aiying Li, Rolf Müller, A Francis Stewart, Jun Fu & Youming Zhang (2016) " RecET direct cloning and Redαβ recombineering of biosynthetic gene clusters, large operons or single genes for heterologous expression. " Nature Protocols 11(7): 1175-90. (通讯作者)
65. Oliver Baker, Ashish Gupta, Mandy Obst, Youming Zhang, Konstantinos Anastassiadis, Jun Fu and A. Francis Stewart (2016) “RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses” Scientific Reports 2016 May 24;6:25529. doi: 10.1038/srep25529. (通讯作者)
66. Papanikos, F., Daniel, K., Goercharn-Ramlal, A., Fei, J.F., Kurth, T., Wojtasz, L., Dereli. I., Fu, J., Penninger, J., Habermann, B., Surani, A., Stewart, A.F. and Toth, A. (2016) "The enigmatic meiotic dense body and its newly discovered component, SCML1, are dispensable for fertility and gametogenesis in mice" Chromosoma. 2016 May 10. [Epub ahead of print]
67. Tu, Q., Yin, J., Fu, J., Herrmann, J., Li, Y., Yin, Y., Stewart, A.F., Müller, R. and Zhang. Y. (2016) "Room temperature electrocompetent bacterial cells improve DNA transformation and recombineering efficiency" Scientific Reports 2016 Apr 20;6:24648. doi: 10.1038/srep24648.
68. Yin, J., Hoffmann, M., Bian, X., Tu, Q., Yan, F., Xia, L., Ding, X., Stewart, A.F., Müller, R., Fu, J., Zhang, Y. （2015）“Direct cloning and heterologous expression of the salinomycin biosynthetic gene cluster from Streptomyces albus DSM41398 in Streptomyces coelicolor A3(2)” Scientific Reports 2015 Oct 13;5:15081. doi: 10.1038/srep15081. (通讯作者)
69. Pan, X., Smith, C.E., Zhang, J., McCabe, K.A., Fu, J., Bell. C.E. (2015) “A structure-activity analysis for probing the mechanism of processive double-stranded DNA digestion by λ exonuclease trimers” Biochemistry 54(39): 6139-48.
70. Wong, F.K., Fei, J.F., Mora-Bermúdez, F., Taverna, E., Haffner, C., Fu, J., Anastassiadis, K., Stewart, A.F., Huttner, W.B. (2015) "Sustained Pax6 Expression Generates Primate-like Basal Radial Glia in Developing Mouse Neocortex" PLoS Biology 08/2015; 13(8):e1002217.
71. Yin, J., Zhu, H., Xia, L., Ding, X., Hoffmann, T., Hoffmann, M., Bian, X., Müller, R., Fu, J., Stewart, A.F., Zhang, Y. (2015) “A new recombineering system for Photorhabdus and Xenorhabdus” Nucleic Acids Research 2015 Mar 31;43(6):e36. doi: 10.1093/nar/gku1336 (通讯作者)
72. Bledau, A. S., Schmidt, K., Neumann, K., Hill, U., Ciotta, G., Gupta, A., Torres, D. C., Fu, J., Kranz, A., Stewart, A. F. and Anastassiadis, K. (2014). "The H3K4 methyltransferase Setd1a is first required at the epiblast stage, whereas Setd1b becomes essential after gastrulation." Development 141: 1022-35.
73. Wang, H., Bian, X., Xia, L., Ding, X., Müller, R., Zhang, Y., Fu, J. and Stewart, A. F. (2014) " Improved seamless mutagenesis by recombineering using ccdB for counterselection " Nucleic Acids Research 2014 Mar;42(5):e37. [Epub ahead of print] (通讯作者)
74. Hu, S., Fu, J., Huang, F., Ding, X., Stewart, A.F., Xia, L. and Zhang, Y. (2013) "Genome engineering of Agrobacterium tumefaciens using the lambda Red recombination system." Applied Microbiology and Biotechnology 2013 Dec 3. [Epub ahead of print]
75. Filipczyk, A., Gkatzis, K., Fu, J., Lickert, H., Anastassiadis, K. and Schroeder, T. (2013) " Biallelic Expression of Nanog Protein in Mouse Embryonic Stem Cells" Cell Stem Cell 13:12-13
76. Bian, X., Fu, J., Plaza, A., Herrmann, J., Pistorius, D., Stewart, A. F., Zhang, Y. and Muller, R. (2013) “In Vivo Evidence for a Prodrug Activation Mechanism during Colibactin Maturation. ” Chembiochem 2013 Jun 6. doi: 10.1002/cbic.201300208. [Epub ahead of print]
77. Thieme, S., Gyárfás, T., Richter, C., Özhan-Kizil, G., Fu, J., Weidinger, G., Buchholz, F., Stewart, A. F., Anastassiadis K. and Brenner S. (2013) “The histone demethylase UTX regulates stem cell migration and hematopoiesis” Blood 121:2462-2473
78. Murdoch, M. B., Owen, N., Stevense, M., Smith, H., Nagaoka, I. S., Hassold, T., Mckay, M., Xu, H., Fu, J., Anastassiadis, K., Revenkova, E., Jessberger, R. and Hunt, P. (2013) “Altered Cohesin Gene Dosage Affects Mammalian Meiotic Chromosome Structure and Behavior” PLOS Genetics 2013 Feb 9(2):e1003241
79. Rostovskaya, M., Naumann, R., Fu, J., Obst, M., Mueller, D., Stewart, A.F. and Anastassiadis, K. (2012) "Transposon mediated BAC transgenesis via pronuclear injection of mouse zygotes." Genesis 2012 Dec 9. doi: 10.1002/dvg.22362. [Epub ahead of print]
80. Rostovskaya, M., Fu, J., Obst, M., Baer, I., Weidlich, S., Wang, H., Smith, A., Anastassiadis, K. and Stewart, A. F. (2012) “Transposon-Mediated BAC Transgenesis in Human ES Cells” Nucleic Acids Research, 2012 Jun 30. [Epub ahead of print] doi: 10.1093/nar/gks643
81. Wojtasz, L., Cloutier, J., Baumann, M., Daniel, K., Varga, J., Fu, J., Anastassiadis, K., Stewart, A. F., Remenyi, A., Turner, J. and Toth, A. (2012) “Meiotic DNA double-strand breaks and chromosome asynapsis in mouse are monitored by distinct HORMAD2-independent and -dependent mechanisms.” Genes & Development 26:958-973
82. Fu, J., Bian, X., Hu, S., Wang, H., Huang, F., Seibert, P., Plaza, A., Xia, L., Müller, R., Stewart, A. F. and Zhang, Y. (2012) "Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting.” Nature Biotechnology 30: 440-446.
83. Bird, A. W., Erler, A., Fu, J., Maresca, M., Heriche, J. K., Zhang, Y., Hyman, A. A. and Stewart, A. F. (2011) "High-efficiency counterselection recombineering for site-directed mutagenesis in bacterial artificial chromosomes." Nature Methods 9:103-109.
84. Nedelkova, M., Maresca, M., Fu, J., Rostovskaya, M., Thiede, C., Anastassiadis, K., Sarov, M. and Stewart, A. F. (2011). "Targeted isolation of genomic regions by recombineering." Nucleic Acids Research 39:e137.
85. Daniel, K., Lange, J., Hached, K., Fu, J., Anastassiadis, K., Roig, I., Cooke, H. J., Stewart, A. F., Wassmann, K., Jasin, M., Keeney, S. and Toth, A. (2011). "Meiotic homologue alignment and its quality surveillance are controlled by mouse HORMAD1." Nature Cell Biology 13:599-610.
86. Skarnes, W. C., Rosen, B., West, A. P., Koutsourakis, M., Bushell, W., Iyer, V., Cox, R., Jackson, D., Severin, J., Biggs, P., Thomas, M., Mujica, A., Harrow, J., Fu, J., Nefedov, M., de Jong, P., Stewart, A. F. and Bradley, A. (2011). "A conditional knockout resource for genome-wide analysis of mouse gene function." Nature 474: 337-42.
87. Ciotta, G., Hofemeister, H., Maresca, M., Fu, J., Sarov, M., Anastassiadis, K. and Stewart, A. F. (2011). "Recombineering BAC transgenes for protein tagging." Methods 53:113-9.
88. Hofemeister, H., Ciotta, G., Fu, J., Seibert, P. M., Schulz, A., Maresca, M., Sarov, M., Anastassiadis, K. and Stewart, A. F. (2010). "Recombineering, transfection, Western, IP and ChIP methods for protein tagging via gene targeting or BAC transgenesis." Methods 53:437-52.
89. Fu, J., Teucher, M., Anastassiadis, K., Skarnes, W. and Stewart, A. F. (2010). "A Recombineering pipeline to make conditional targeting constructs." Methods in Enzymology 477C: 125-144.
90. Maresca, M., Erler, A., Fu, J., Friedrich, A., Zhang, Y. and Stewart, A. F. (2010). "Single-stranded heteroduplex intermediates in Lambda Red homologous recombination." BMC Molecular Biology 11: 54.
91. Kranz, A., Fu, J., Duerschke, K., Weidlich, S., Naumann, R., Stewart, A. F. and Anastassiadis, K. (2010). "An improved Flp deleter mouse in C57Bl/6 based on Flpo recombinase." Genesis 48:512-520.
92. Anastassiadis, K., Fu, J., Patsch, C., Hu, S., Weidlich, S., Duerschke, K., Buchholz, F., Edenhofer, F. and Stewart, A. F. (2009). "Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice." Disease Models & Mechanisms 2: 508-15.
93. Fu, J., Wenzel, S. C., Perlova, O., Wang, J., Gross, F., Tang, Z., Yin, Y., Stewart, A. F., Müller, R. and Zhang, Y. (2008). "Efficient transfer of two large secondary metabolite pathway gene clusters into heterologous hosts by transposition." Nucleic Acids Research 36: e113.
94. Erler, A., Maresca, M., Fu, J. and Stewart, A. F. (2006). "Recombineering reagents for improved inducible expression and selection marker re-use in Schizosaccharomyces pombe." Yeast 23: 813-23.
95. Gross, F., Ring, M. W., Perlova, O., Fu, J., Schneider, S., Gerth, K., Kuhlmann, S., Stewart, A. F., Zhang, Y. and Müller, R. (2006). "Metabolic engineering of Pseudomonas putida for methylmalonyl-CoA biosynthesis to enable complex heterologous secondary metabolite formation." Chemistry & Biology 13: 1253-64.
96. Perlova, O., Fu, J., Kuhlmann, S., Krug, D., Stewart, A. F., Zhang, Y. and Müller, R. (2006). "Reconstitution of the myxothiazol biosynthetic gene cluster by Red/ET recombination and heterologous expression in Myxococcus xanthus." Applied and Environmental Microbiology 72: 7485-94.
97. Wang, J., Sarov, M., Rientjes, J., Fu, J., Hollak, H., Kranz, H., Xie, W., Stewart, A. F. and Zhang, Y. (2006). "An improved recombineering approach by adding RecA to lambda Red recombination." Molecular Biotechnology 32: 43-53.
98. Wenzel, S. C., Gross, F., Zhang, Y., Fu, J., Stewart, A. F. and Müller, R. (2005). "Heterologous expression of a myxobacterial natural products assembly line in pseudomonads via Red/ET recombineering." Chemistry & Biology 12: 349-56.
国际专利
1. 张友明、王海龙、符军、A. Francis Stewart（08.2017）基因组大片段直接克隆和DNA多分子组装新技术，国际发明专利申请号： PCT/CN2017/000483
2. STEWART, Adrian, Francis; FU, Jun; EHRHARDT, Anja; EHRKE-SCHULZ, Eric; ZHANG, Wenli “Adenoviral vectors” PCT/EP2017/058306
3. TU, Qiang; MÜLLER, Rolf; STEWART, Francis; FU, Jun; ZHANG, Youming. “High efficiency of electrocompetent cells prepared at room temperature” PCT/EP2013/071546
4. FU, Jun; ZHANG, Youming; STEWART, Francis. “Direct cloning of DNA fragment from genomic DNA without library construction and screening” PCT/IB2011/052549
5. ZHANG, Youming; FU, Jun; BIAN, Xiaoying; STEWART, Francis; ZIPS, Daniel; MÜLLER, Rolf “Heterologous expression of PKS/NRPS gene cluster and targeted anticancer drug delivery.” PCT/IB2010/055923
6. MARESCA, Marcello; ERLER, Axel, Steffen; FU, Jun; SEIBERT, Philipp, Martin; STEWART, Adrian, Francis; ZHANG, Youming “Method of nucleic acid recombination” PCT/IB2009/000488
7. ZHANG, Youming; MÜLLER, Rolf; STEWART, Francis; GROSS, Frank; WENZEL,Silke, C.; FU, Jun “Methods for heterologous expression of secondary metabolites” PCT/IB2005 /003650; EP1809749 (2007); US20090298117 (2009)
中国专利
授权专利：
8. 张友明、尹佳、符军、郑文韬。假单胞菌中新型重组系统及其应用，中国发明专利申请号：CN201710313101.8（申请日：2017.05.05），授权专利号：ZL201710313101.8（授权日：2019.12.13）
9. 张友明、王海龙、符军、A.F.斯图尔特。基因组大片段直接克隆和DNA多分子组装新技术，中国发明专利：CN201710177676.1 （申请日：2017.03.23），授权号：ZL201710177676.1（授权日：2020.06.16）
10. 马静云，符军，郑瑶瑶，袁恒星，王海龙，张友明。表达猪繁殖与呼吸综合征病毒GP3/GP5/M基因的重组伪狂犬病毒、构建方法和应用，中国发明专利申请号：CN202010647705.8（申请日：2020.07.07），授权专利号：ZL202010647705.8（授权日：2021.10.08）
11. 王海龙，宋超逸，符军，张友明。一种含有多杀菌素多操纵子人工基因簇质粒的构建方法与应用，中国发明专利申请号：CN201811407515.8（申请日：2018.11.23），授权专利号：ZL201811407515.8（授权日：2021.10.22）
12. 马静云，燕晓玲，符军。一种猪急性腹泻综合征冠状病毒人工染色体重组载体及其构建方法和应用，中国发明专利申请号：CN202210559369.0（申请日：2022.05.23），授权专利号：ZL202210559369.0（授权日：2022.08.02）
13. 符军， 李瑞娟， 史洪波，赵晓雨，张友明。伯克氏菌同源重组系统及其应用，中国发明专利申请号：CN202011131375.0（申请日：2020.10.21），授权专利号：ZL 202011131375.0（授权日：2022.08.26）
14. 王海龙、宋超逸、符军、张友明。 一种大型基因簇的无痕定点改造方法及其应用，中国发明专利申请号：CN202010147911.2（申请日：2020.03.05），授权专利号：ZL202010147911.2（授权日：2023.01.24）
15. 谢青梅; 封柯宇; 符军; 邵冠明; 张新珩。一种预防台湾型传染性支气管炎的疫苗株，中国发明专利申请号：CN201911161598.1（申请日：2019.11.22），授权专利号：ZL201911161598.1（授权日：2023.05.19）
16. 李瑞娟，宫恺，王茂芹，宋超逸，张友明，符军，李爱英。一株高产FK228的工程菌株及其构建与应用，中国发明专利申请号：CN202210109315.4.（申请日：2022.09.15），授权专利号：ZL202210109315.4（授权日：2023.09.15）
17. 符军，李瑞娟，边志龙，李珊珊，杨润雨，涂强，张友明。农杆菌同源重组系统及其应用，中国发明专利申请号：CN202111088227.2（申请日：2021.09.16）授权专利号：ZL202111088227.2（授权日：2024.01.16）
18. 李瑞娟，宋超逸，王茂芹，宫恺，张友明，符军，李爱英。化合物Spiruchostatin E及其制备方法和应用，中国发明专利申请号：CN202211121904.0（申请日：2022.09.15）授权专利号：ZL202211121904.0（授权日：2024.08.27）
19. 李瑞娟，宫恺，张友明，王茂芹，宋超逸，符军，李爱英。一组FK228衍生物及其制备方法与应用，中国发明专利申请号：CN202211122305.0（申请日：2022.09.15）授权专利号：ZL202211122305.0（授权日：2024.11.15）
20. 李瑞娟，雍道敬，倪金凤，符军，涂强，张友明。中国发明专利，名称：一种链霉菌sdu1201和或其次级代谢产物在防治植物害虫中的应用。发明专利申请号：CN202411618710.0（申请日：2024.11.13）授权专利号：ZL202411618710.0（授权日：2025.12.26）
21. 李瑞娟，李悦，封雪艳，谢卫东，李爱英，符军，张友明。链霉菌AH1901-2及其应用、萘霉素类化合物的制备方法及应用。专利申请号：CN202411872722.6（申请日期：2024.12.18）授权专利号：ZL202411872722.6（授权日：2026.03.25）
实质审查阶段专利：
22. 谢青梅; 封柯宇; 符军; 邵冠明; 张新珩。一种快速制备流行性传染性支气管炎疫苗的方法，中国发明专利申请号：CN201911161596.2（申请日：2020.11.05）
23. 谢青梅; 符军; 何家辉; 刘仙琦; 封柯宇; 邵冠。一种重组3型鸭腺病毒、制备方法及其应用，中国发明专利申请号：CN202210179819.3（申请日：2022.02.25）
24. 尹佳，曹羽欣，符军，李瑞娟。中国发明专利，名称：一株戊糖片球菌及其应用。中国发明专利申请号：CN202311822789.4（申请日：2023.12.27）
25. 符军，李瑞娟，段琼，倪金凤，李爱英，张友明。中国发明专利，名称：一种脂肽类化合物及其制备方法与应用。发明专利申请号：CN202410952553.0（申请日：2024.07.16）
26. 符军，李瑞娟，王秀玲，张友明。中国发明专利，名称：DNA修饰与转移一体化大肠杆菌工程菌株及其构建方法。发明专利申请号：CN202411058039.9（申请日：2024.08.02）
27. 符军，李瑞娟，王秀玲，张友明。中国发明专利，名称：链霉菌中外源基因簇染色体整合体系的构建方法及其应用。发明专利申请号：CN202411054594.4（申请日：2024.08.02）
28. 符军，李瑞娟，杨润雨，张友明。中国发明专利，名称：肺炎克雷伯菌同源重组系统及其应用。发明专利申请号：CN202411243101.1（申请日：2024.09.05）
29. 李瑞娟，雍道敬，倪金凤，符军，涂强，张友明。中国发明专利，名称：一株灰色链霉菌H3950及其应用。发明专利申请号：CN202411611230.1（申请日：2024.11.12）
30. 符军，李瑞娟，高诗情，许守英，涂强，张友明。中国发明专利，名称：根癌农杆菌基因组编辑系统及其构建方法与应用。发明专利申请号：CN202411660758.8（申请日：2024.11.20）
31. 李瑞娟，符军，马骏驰，王茂芹。化合物FK228 D及其制备方法和应用。专利申请号：CN202610127023.1（申请日期：2026.01.29）
32. 李瑞娟，符军，邓运佳，李爱英，张友明。一种波氏杆菌基因组编辑系统及其构建方法与应用。专利申请号：2026103020160，申请日期：2026.03.12。
33. 李瑞娟，李爱英，邓运佳，李振，宋晓飞，符军，张友明。一种巴氏杆菌同源重组系统及其应用。专利申请号：2026103020156，申请日期：2026.03.12。
