1）从表型到组学研究(基因组/表观遗传组/肠道菌群组等），多维度探索环境与生殖健康的关系，实现宏观与微观证据互相佐证；2）通过大型医学登记资料探索围产期疾病/用药对子代出生缺陷及远期发育的长期影响，促进早期预防的理论和实践；3）围绕与人类生殖过程密切相关的重要生理事件如精子发生，胚胎发育，出生结局，婴幼儿发育等开展系列研究，为当前日益严峻的生殖相关问题的解决提供重要的策略方向。

多维度探索环境与生殖健康的关系，实现宏观与微观证据互相佐证；在国际上首次报道了BPA导致男性精液质量下降、精子运动轨迹改变、性功能改变及成年男性/女性生殖激素异常，并可干扰人类青春发育启动与进程；同时全面评估了孕期双酚类化合物、溴系阻燃剂、有机磷类阻燃剂、全氟/多氟烷基化合物等一系列污染物对子代出生结局、生殖、体格和神经发育的不良影响，多项为首次报道。率先提出环境污染物暴露可引起精子、胎盘DNA甲基化/羟甲基化水平变化，并发现了表观遗传学改变在环境污染物人类生殖毒性中的介导作用；进一步联合转录组、代谢组等多组学分析技术阐明了环境污染物人类生殖毒性的表观遗传作用机制，为孕期环境污染物暴露影响子代长期健康的编程假说提供了科学依据。 系列研究基于大样本前瞻性的研究设计，结合了内外暴露评估指标，所涉多个暴露-结局关联证据及表观遗传通路互相支撑、彼此佐证，为世界主要国家制定或修订相关化合物使用标准提供了直接证据，被Science等知名学术期刊以及欧洲食品安全局、美国环境保护署等国际机构广泛引用，在国际范围内产生了显著的社会和政策效应。2）基于北欧医学登记资料探索围产期疾病/用药对子代出生缺陷及远期发育的长期影响。对父亲抗抑郁药、抗癫痫药、母亲beta 2受体激动剂用药、母亲多囊卵巢综合症等对子代发育的影响进行了系统研究。部分代表性成果如下：

1) Zhenzhen Xie#, Longmei Jin#, Qihan Wu, Honglei Ji, Maohua Miao, Xiuxia Song, Haijun Zhu,  Huijia Su, Wei Yuan*, Hong Liang*. Maternal per- and polyfluoroalkyl substance concentrations and placental DNA methylation of thyroid hormone-related genes. Journal of Hazardous Materials. 2025 Feb 8:489:137545. doi: 10.1016/j.jhazmat.2025.137545. Online ahead of print.

2) Jiaxian Chen, Maohua Miao, Xiuxia Song, Honglei Ji, Hongchao Lian, Yao Chen, Wei Yuan*, Ziliang Wang*. Tracing impactJiaxian Chen, Maohua Miao, Xiuxia Song, Honglei Ji, Hongchao Lian, Yao Chen, Wei Yuan*, Ziliang Wang*. Tracing impacts of prenatal exposure to bisphenol analogues on child anogenital distance development: A birth-cohort study. Journal of Hazardous Materials. 2025 Feb 27:490:137730. doi: 10.1016/j.jhazmat.2025.137730. Online ahead of print.

3) Fen Yang #, Ziliang Wang #, Henrik Toft Sørensen, Imre Janszky, Mika Gissler, Wei Yuan, Maohua Miao, Nathalie Roos , Anna-Karin Wikström, Jiong Li, Krisztina D László. Maternal polycystic ovary syndrome and Offspring's Risk of Cardiovascular diseases in Childhood and Young Adulthood. Nature Communications. 2024 Nov 29;15(1):10414. doi: 10.1038/s41467-024-54795-w.                                

4) Zhenzhen Xie, Hong Liang, Maohua Miao, Ziliang Wang, Yao Chen, Lan Yang, Yan Zhou, Wencheng Cao, Wei Yuan*. Prenatal Exposure to Perfluoroalkyl Substances and Cognitive and Neurobehavioral Development in Children at 6 Years of Age. Environmental Science & Technology. 2023 Jun 6;57(22):8213-8224. doi: 10.1021/acs.est.2c06535. Epub 2023 May 22.    

5) Min Luan*, Fen Yang, Maohua Miao, Wei Yuan, Mika Gissler, Elizabeth V Arkema, Donghao Lu, Jiong Li, Krisztina D László. Rheumatoid arthritis and the risk of postpartum psychiatric disorders: a Nordic population-based cohort study. BMC Medicine. 2023 Apr 3;21(1):126. doi: 10.1186/s12916-023-02837-3.  

6)  Lan Yang,# Yao Chen,# Honglei Ji, Xi Zhang, Yan Zhou, Jianhui Li, Yan Wang, Zhenzhen Xie, Wei Yuan, Hong Liang,* Maohua Miao. Per- and Poly-fluoroalkyl Substances and Bile Acid Profiles in Pregnant Women. Environmental Science and Technology. 2023 Oct 24;57(42):15869-15881. doi: 10.1021/acs.est.3c05106. Epub 2023 Oct 11.          

7) Ziliang Wang, Maohua Miao, Jianhua Xu, Yafei Chen, Hong Liang, Lan Yang, Xiao Liu, Sheng Wen，Xiaowen Tu*, Wei Yuan*. Gestational exposure to bisphenol analogues and kisspeptin levels in pregnant women and their children: A pregnancy-birth cohort study. Science of the Total Environment. 2022 Nov 20:848:157720. doi: 10.1016/j.scitotenv.2022.157720. Epub 2022 Jul 29.

8)  Min Luan, Hong Liang, Yafei Chen, Da Chen, Honglei Ji, Hexia Chen, Maohua Miao*, Wei Yuan. Prenatal exposure to organophosphate esters is associated with decreased anogenital distance in offspring. Science of the Total Environment. 2023 Jan 15;856(Pt 1):159050. doi: 10.1016/j.scitotenv.2022.159050. Epub 2022 Sep 26.

9) Xiaowei Sun, Cong Liu, Hong Liang, Maohua Miao, Ziliang Wang, Honglei Ji, Aaron van Donkelaar, Randall V. Martin, Haidong Kan*, Wei Yuan. Prenatal exposure to residential PM2.5 and its chemical constituents and weight in preschool children: A longitudinal study from Shanghai, China. Environment International. 2021 Sep;154:106580. doi: 10.1016/j.envint.2021.106580. Epub 2021 Apr 24.                                                              

Honglei Ji, Hong Liang, Yongfu, Ziliang Wang, Wei Yuan, Xu Qian, Ellen Margrethe Mikkelsen, Anne Sofie Dam Laursen, GuangHong Fang, Guoying Huang*, Maohua Miao*, Jiong Li. Association of Maternal History of Spontaneous Abortion and Stillbirth With Risk of Congenital Heart Disease in Offspring of Women With vs Without Type 2 Diabetes. JAMA Netw Open. 2021 Nov 1;4(11):e2133805.doi: 10.1001/jamanetworkopen.2021.33805.